CGSC Strain#: 5653
Strain Designation: C84     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510,
pho-30Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- pho-30-- is either phoS or phoT.
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Garen, A., S. Garen 1963. Genetic evidence on the nature of the repressor for alkaline phosphatase in E. coli. J.Mol.Biol. 6:433-438