CGSC Strain#: 5643
Strain Designation: RS5065      Source of Strain: B. Konrad
Sex: F- Chromosomal Markers: λ^-, trpA33, IN(rrnD-rrnE)1, polA546(ts,EX)
Strain Comments:

• trpA33-- has two consecutive nucleotide substitutions (R.Milkman) and has not been known to revert (C.Yanofsky via R.Milkman).
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• polA546(ts,EX)-- was called polAex2 by Konrad and Lehman. It is a temperature-sensitive mutation, polA546, EX TS. Ref to polA5: Grindley & Kelley 1976, Mol.Gen.Genet. 143:311.
• EX = exonuclease activity mutation
• ts = Temperature Sensitive

References:

• Konrad, EB, IR Lehman 1974. A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I. Proc. Natl. Acad. Sci. U.S.A. 71(5):2048-51.
• Grindley, ND, WS Kelley 1976. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143(3):311-8.