CGSC Strain#: 5587
Strain Designation: 58      Source of Strain: Gray & Tatum
Sex: F+ Chromosomal Markers: bio-1(Unst), spoT1, creC510
Strain Comments:

• Ref. for phoM510: See Wanner, 1987, cited below.
• bio-1(Unst)-- : This marker apparently reverts at high frequency
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• Unst = unstable

References:

• Gray, A., E. L. Tatum 1944. X-ray induced growth factor requirements in bacteria. Proc.Natl.Acad.Sci.USA 30:404-410
• Tatum, E. L. 1945. X-ray induced mutant strains of Escherichia coli. Proc.Natl.Acad.Sci.USA 31:215-219
• Tatum, E. L., J. Lederberg 1947. Gene recombination in the bacterium. J.Bacteriol. 53:673-684
• Lederberg, J 1949. Aberrant Heterozygotes in Escherichia Coli. 35(4):178-84.
• Wanner, B.L. 1987. Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region. J.Bacteriol. 169:900-903