CGSC Strain#: 7599
Strain Designation: YYC186      Source of Strain: J.E. Cronan
Sex: F- Chromosomal Markers: Δ(aroP-aceF)73, zac-284::Tn10, lacZ608(Am), λ^-
Strain Comments:

• This strain is grown on Luria plus acetate.
• zac-284::Tn10-- cotransduces ca. 99% with aceEF; in YYC100, see comment about ts mutation, not present in YYC186.
• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• = tetracycline resistant
• Am = amber(UAG) mutation

References:

• Chang, Y.-Y., J.E. Cronan 1982. Mapping nonselectable genes of Escherichia coli by using transposon Tn10 location of a gene affecting pyruvate oxidase. J.Bacteriol. 151:1279-1289
• Chang, Y.-Y., J.E. Cronan 1988. Common ancestry of Escherichia coli pyruvate oxidase and the acetohydroxy acid synthases of the branched-chain amino acid biosynthetic pathway. J.Bacteriol. 170:3937-3945