CGSC Strain#: 5513
Strain Designation: Lin 89     
Source of Strain: E.C.C. Lin
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
glpR12(glpn),
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - glpR12(glpn)-- is a mutation which rendered the parent strain non-inducible.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- glpn = non-inducible for glp regulon
- T2R = T2 Resistant
References: - Cozzarelli, NR, WB Freedberg, EC Lin 1968. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J. Mol. Biol. 31(3):371-87.