CGSC Strain#: 5518
Strain Designation: JRG301      Source of Strain: J.R. Guest
Other Designations: T3A48lpd1
Sex: F- Chromosomal Markers: lpd-1, λ^-, trpA58, trpE61
Strain Comments:

• This strain was formerly called T3A58lpd1. It might also carry a trpR mutation.
• lpd-1-- : add glucose to LB or other complete media; add 2 mM acetate and 2 mM succinate to glucose minimal media. Glucose-free media will select against the mutation.
• = missense

References:

• Guest, JR, IT Creaghan 1973. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and characterization of lipoamide dehydrogenase mutants. J. Gen. Microbiol. 75(1):197-210.
• Guest, JR, IT Creaghan 1974. Further studies with lipoamide dehydrogenase mutants of Escherichia coli K12. J. Gen. Microbiol. 81(1):237-45.
• Guest, JR 1974. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: Chromosomal location of the lipoamide dehydrogenase gene. J. Gen. Microbiol. 80(2):523-32.