CGSC Strain#: 5535
Strain Designation: PS1518     
Source of Strain: Bryant & Sypherd
Sex: F-
Chromosomal Markers: lacY1 or lacZ4,
glnX44(AS)
,
gal-6,
λ-,
his-99,
rpsL8 or rplL9(L?)
or rpsL17,
malT1(λ
R)
,
ilvD16,
metB1,
ΔargH1,
thiE1,
rimA1(Cs)
,
pro-52Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- rimA1(Cs)-- is a cold-sensitive mutation. The permissive temperature is 37 degrees C, the restrictive temp. is 25 degrees C.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- = strep-resistant
- AS = amber(UAG) suppressor
- Cs = cold-sensitive
- LamR = Lambda-resistant
References: - Bryant, RE, PS Sypherd 1974. Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli. J. Bacteriol. 117(3):1082-92.