CGSC Strain#: 5702
Strain Designation: F
-EC-O,rif180,strA     
Source of Strain: Chakrabarti & Gorini
Sex: F-
Chromosomal Markers: Δ(gpt-lac)5,
glnX44(AS)
,
λ-,
relA1,
rpsL124(strR)
,
rpoB180,
thiE1Strain Comments: - Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- strR = streptomycin resist.
References: - Chakrabarti, SL, L Gorini 1975. A link between streptomycin and rifampicin mutation. Proc. Natl. Acad. Sci. U.S.A. 72(6):2084-7.
- Chakrabarti, SL, L Gorini 1977. Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination. Proc. Natl. Acad. Sci. U.S.A. 74(3):1157-61.