CGSC Strain#: 5808
Strain Designation: M7032      Source of Strain: J.R. Beckwith
Sex: F- Chromosomal Markers: lacZ625(Am), λ^-, relA1, rpsL150(strR), spoT1
Strain Comments:

• lacZ625(Am)-- comes from strain Hfr 3000 YA625. It is an amber mutation, in `interval 14' of Newton et al. JMB14:290(1965).
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296