CGSC Strain#: 5811
Strain Designation: M7048      Source of Strain: J.R. Beckwith
Sex: F- Chromosomal Markers: lacZ659(Oc), λ^-, relA1, rpsL150(strR), spoT1
Strain Comments:

• lacZ659(Oc)-- is the allele from D. Zipser strain NG659. It is an ochre mutation, in `interval 15' of Newton et al.JMB14:290(1965).
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296