CGSC Strain#: 7687
Strain Designation: CY15011      Source of Strain: C. Yanofsky
Sex: F- Chromosomal Markers: λ^-, trpL-729, IN(rrnD-rrnE)1, rph-1, tnaA5, rpoB2066, trpR55
Strain Comments:

• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• rpoB2066 was formerly called rpoB6
• tnaA5 was formerly called tna-2
• trpL-729 was formerly called trpL29

References:

• Yanofsky, C, V Horn 1981. Rifampin resistance mutations that alter the efficiency of transcription termination at the tryptophan operon attenuator. J. Bacteriol. 145(3):1334-41.