CGSC Strain#: 7692
Strain Designation: W3110tnaA2trpEFBR19     
Source of Strain: C. Yanofsky
Sex: F-
Chromosomal Markers: λ-,
trpE719(fbR)
,
IN(rrnD-rrnE)1,
rph-1,
tnaA5Strain Comments: - IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- tnaA5 was formerly called tna-2
- trpE719(fbR) was formerly called trpEFBR19
- trpE719(fbR) was formerly called trpE19FBR
- fbR = feedback resistant
References: - Doolittle, W.F., C. Yanofsky 1968. Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase. J.Bacteriol. 95:1283-1294
- Yanofsky, C., R.L. Kelley, V. Horn 1984. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe. J.Bacteriol. 158:1018-1024