CGSC Strain#: 7852
Strain Designation: AG100kan      Source of Strain: S.B. Levy
Sex: F- Chromosomal Markers: glnX44(AS), galK2(Oc), Δ(marR-marB)876(::kan), rpsL704(strR), xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = kanamycin resistant
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Maneewannakul, K, SB Levy 1996. Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli. Antimicrob. Agents Chemother. 40(7):1695-8.