BD792      Source of Strain:
F- Chromosomal Markers: λ-
, rph-1Strain Comments:
- This is an F- derivative of W1485, cured with acridine orange. We received reports that this strain is Fnr- and our tests of our working culture confirmed that in 2003. Cultures from lyophil tested Fnr+ and our current culture is from a lyophil.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- Am = amber(UAG) mutation