CGSC Strain#: 6156
Strain Designation: UT3062      Source of Strain: C. Lark
Sex: F- Chromosomal Markers: trpE68, thyA42, rpsL261(strR), metE101, deoB20, arg-69
Strain Comments:

• This strain is a derivative of E. coli strain 15.
• thyA42-- E. coli 15 mutation
• thyA42-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deoB20-- E. coli 15 mutation.
• deoB20 was formerly called thyR32
• strR = streptomycin resist.

References:

• Lark, CA, J Riazi, KG Lark 1978. dnaT, dominant conditional-lethal mutation affecting DNA replication in Escherichia coli. J. Bacteriol. 136(3):1008-17.