CGSC Strain#: 6192
Strain Designation: DF1010     
Source of Strain: D. Fraenkel
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
ompF627(T2R)
,
ΔpfkB201,
fadL701(T2R)
,
recA56,
relA1,
pitA10,
spoT1,
Δ(rhaD-pfkA)200,
rrnB-2,
mcrB1,
creC510Strain Comments: - recA56-- Amino acid substitution Arg 60 Cys
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Daldal, F. 1983. Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme. J.Mol.Biol. 168:285-305