CGSC Strain#: 7836
Strain Designation: BW23322     
Source of Strain: B.L. Wanner
Sex: F-
Chromosomal Markers: Δ(argF-lac)169,
ΔuidA4::pir-116,
rpoS396(Am)
,
endA9(del-ins)
::FRT,
rph-1,
hsdR514,
rob-1,
creC510Strain Comments: - Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- ΔuidA4::pir-116-- Allows plasmids with an R6Kγ origin to replicate to high copy number
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- endA9(del-ins)::FRT was formerly called endABT333
- Am = amber(UAG) mutation
- Const = constitutive
- del-ins = deletion-insertion
References: