CGSC Strain#: 7893
Strain Designation: DPB924      Source of Strain: D.P. Biek
Sex: F- Chromosomal Markers: λ^-, rph-1
Strain Comments:

• topA⁺ sibling of DPB923.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• Biek, D.P., S.N. Cohen 1989. Involvement of integration host factor (IHF) in maintenance of plasmid pSC101 in Escherichia coli: mutations in the topA gene that allow pSC101 replication in the absence of IHF. J.Bacteriol. 171:2066-2074