CGSC Strain#: 6761
Strain Designation: BW6166      Source of Strain: B.L. Wanner
Sex: Hfr    Point of Origin#: 18   Map Position: 91.46   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: glnX44(AS), λ^-, glgP721::Tn10, thiE1, malK16(λ^R)
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glgP721::Tn10-- : originated in BW6166 as insertion ca. 16 min. from PO18 of J4; shown by PCR to be within glgP gene, at nucleotide 3,564,175 (76.827 min.)
• malK16(λ^R)-- F is integrated in the malKgene of the malB region.
• glgP721::Tn10 was formerly called zhh-721::Tn10
• glgP721::Tn10 was formerly called zhh-21::Tn10
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = tetracycline resistant
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Wanner, B.L. 1986. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J.Mol.Biol. 191:39-58