CGSC Strain#: 7896
Strain Designation: XA106      Source of Strain: J.H. Miller
Sex: F- Chromosomal Markers: Δ(gpt-lac)5, λ^-, ara(FG), gyrA-0(NalR), metB-, argE-(Am), rpoB0(rifR), thiE1, leuX151(AS)
Strain Comments:

• See also Promega Technical Bulletin on Interchange System.
• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
• leuX151(AS)-- Su+6(supP) is a double mutant of a leu tRNA species . anticodon CAA to CUA and at junction of D- and anticodon-stem (A27 to G27).
• leuX151(AS) was formerly called Su6⁺
• leuX151(AS) was formerly called supP51
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• rifR = rifamycin resistance

References:

• Miller, J.H., D. Ganem, P. Lu, A. Schmitz 1977. Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map. J.Mol.Biol. 109:275-301