CGSC Strain#: 7897
Strain Designation: XA105      Source of Strain: J.H. Miller
Sex: F- Chromosomal Markers: Δ(gpt-lac)5, lysT47(AS*), λ^-, ara(FG), gyrA-0(NalR), metB-, argE-(Am), rpoB0(rifR), thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS* = synthetic amber suppressor mut
• NalR = Nalidix.acidResist.
• rifR = rifamycin resistance

References:

• Miller, J.H., D. Ganem, P. Lu, A. Schmitz 1977. Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map. J.Mol.Biol. 109:275-301
• Normanly, J., L.G. Kleina, J.M. Masson, J. Abelson 1990. Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J.Mol.Biol. 213:719-726