CGSC Strain#: 6859
Strain Designation: WL2     
Source of Strain: Lorowitz & Clark
Sex: F-
Chromosomal Markers: fadR201,
tyrT58(AS)
,
adhE2,
adhEp-81,
mel-1Strain Comments: - adhE2-- Little or no ADH activity under aerobic or anaerobic conditions; reduced ACDH (acetaldehyde CoA deHase) activity. Allyl alcohol resistance in adhEp81 background.
- adhEp-81-- is an operator or promoter mutation. It results in 60-fold overproduction of ADH on succinate plus acetate medium and allows growth on EtOH as sole C source.
- adhEp-81 was formerly called adhC81
- tyrT58(AS) was formerly called supF58
- AS = amber(UAG) suppressor
References: - Lorowitz, W., D.P. Clark 1982. Mutants of Escherichia coli with a temperature-sensitive alcohol dehydrogenase. J.Bacteriol. 152:935-938