CGSC Strain#: 8210
Strain Designation: BW21038/pAH34      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pAH34
   Plasmid Markers/Mutations: oriR6K_γ, MCS, oriT_RP4, bla-1(ApR,dPstI), ori_f1,+, lacZα
   Plasmid Comment: Differs from pWM71 by removal of PstI site in {\it bla} gene, so generally more useful than pWM71.
Chromosomal Markers: Δ(codB-lacI)3, ΔphoA532, ΔuidA3::pir⁺, recA1, rph-1, Δ(phnP-phnD)3330( phnC?), mini-Tn5Lac4(CamR,lacI^Q)
Strain Comments:

• pAH34 is like pWM71 except for deletion of PstI site in bla. pWM71 host is pir-116 and has F128-Tn10-11.
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• ΔuidA3::pir⁺-- Allows the replication of plasmids with an R6Kγ origin of replication
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• ΔuidA3::pir⁺ was formerly called uidA(Δ MluI)::pir⁺
• CamR = chloramphenicol resistant
• phnC? = may also delete phnC
• ApR = ampicillin resistant
• dPstI = PstI site deleted

References:

• Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.