CGSC Strain#: 6975
Strain Designation: N3431      Source of Strain: D. Apirion
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ43(Fs), λ^-, rne-3071(ts), relA1, spoT1, thiE1
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Fs = Frameshift mutation
• ts = Temperature Sensitive

References:

• Apirion, D. 1978. Isolation, genetic mapping, and some characterization of a mutation in Escherichia coli which affects the processing of ribonucleic acid. Genetics 90:659-671
• Goldblum, K, D Apririon 1981. Inactivation of the ribonucleic acid-processing enzyme ribonuclease E blocks cell division. J. Bacteriol. 146(1):128-32.