CGSC Strain#: 5250
Strain Designation: H5a     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
phoB54,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
phoT-35,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- phoT-35-- is a phoS or phoT mutation.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Kreuzer, K.N., C. Pratt, A. Torriani 1975. Genetic analysis of regulatory mutants of alkaline phosphatase of Escherichia coli. Genetics 81:459-468