CGSC Strain#: 8133
Strain Designation: BW27876      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(citA-citB)1289, λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
• ΔlacZ4787(::rrnB-3) was formerly called Δ lacZ_WJ16
• ::rrnB-3 = 4 copies of rrnB inserted

References:

• Oshima, T., H. Aiba, Y. Masuda, S. Kanaya, M. Sugiura, B.L. Wanner, H. Mori, T. Mizuno 2002. Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12. Mol.Microbiol. 46:281-291
• Zhou, L., X.H. Lei, B.R. Bochner, B.L. Wanner 2003. Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems. J.Bacteriol. 185:4956-49572