CGSC Strain#: 4961
Strain Designation: W3110 ColE1     
Source of Strain: D. Helinski
Sex: F-
Episome/Plasmid: ColE1
Plasmid Markers/Mutations: Plasmid Comment: For pLC's, colicin E1 can be used for selection -- 1 unit/ml. media of commercial prep (e.g., Sigma) gave good amplification (see feedback, Req. schmitt) or 1/100-1/1000 dil. of lysate of ColE1 strain
Chromosomal Markers: λ-,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
References: - Herschman, H.R., D. Helinski 1967. Comparative study of the events associated with colicin induction. J.Bacteriol. 94:691-699