CGSC Strain#: 4976
Strain Designation: Hfr 3000 YA14      Source of Strain: F. Jacob
Other Designations: 3000Y14
Other Designations: CA85
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ53(Am), λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation

References:

• Borek, E., A. Ryan, J. Rockenbach 1955. Nucleic acid metabolism in relation to the lysogenic phenomenon. J.Bacteriol. 69:460-467
• Signer, E., J.R. Beckwith, S. Brenner 1965. Mapping of suppressor loci in Escherichia coli. J.Mol.Biol. 14:153-166