CGSC Strain#: 4881
Strain Designation: AM1     
Source of Strain: D. Fraenkel
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
pfkA1,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Morrissey, AT, DG Fraenkel 1968. Selection of fructose 6-phosphate kinase mutants in Escherichia coli. Biochem. Biophys. Res. Commun. 32(3):467-73.
- Morrissey, AT, DG Fraenkel 1969. Chromosomal location of a gene for fructose 6-phosphate kinase in Escherichia coli. J. Bacteriol. 100(2):1108-9.