CGSC Strain#: 7710
Strain Designation: AW824      Source of Strain: J. Adler
Sex: F- Chromosomal Markers: relA1, spoT1, fad-3165::Tn10kan, metB1, rrnB-2, mcrB1, creC510
Strain Comments:

• Revertant of hemG mutant AW824, with no accumulation of porphyrins, and no tumbling response to blue light. Control for AW823.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• = kanamycin resistant
• Const = constitutive

References:

• Yang, H., A. Sasarman, H. Inokuchi, J. Adler 1996. Non-iron porphyrins cause tumbling to blue light by an Escherichia coli mutant defective in hemG. Proc.Natl.Acad.Sci.USA 93:2459-2463