CGSC Strain#: 8073
Strain Designation: MGM100     
Source of Strain: M. Masters
Sex: F-
Chromosomal Markers: λ-,
rph-1,
araBADp-groE,
zje-2335::kanStrain Comments: - The groE promoter has been replaced by araBADp and the kan insertion follows the groS, groL genes.
- The strain may also be Fnr-minus.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
References: - McLennan, N, M Masters 1998. GroE is vital for cell-wall synthesis. Nature 392(6672):139-.
- Nielsen, KL, N McLennan, M Masters, NJ Cowan 1999. A single-ring mitochondrial chaperonin (Hsp60-Hsp10) can substitute for GroEL-GroES in vivo. J. Bacteriol. 181(18):5871-5.