CGSC Strain#: 4383
Strain Designation: DS100      Source of Strain: D.A. Steege
Sex: F- Chromosomal Markers: pro-48, tsx-84, LAMcI857(ts), LAMdsu1+, trpA9605(Am), his-85(Am), trpR55, ilv-632
Strain Comments:

• This strain should be grown at 30 degrees C on medium containing Ilv and Pro and lacking Tryp and His.
• pro-48-- is probably in proB^-.
• LAMdsu1+-- carries the mutant allele serU32(supD32), amber suppressor.
• trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
• his-85(Am)-- is an amber mutation. It is suppressed by supD32.
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• his-85(Am) was formerly called his-29
• Am = amber(UAG) mutation
• ts = Temperature Sensitive

References:

• Steege, DA, B Low 1975. Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli. J. Bacteriol. 122(1):120-8.