CGSC Strain#: 4422
Strain Designation: W677     
Source of Strain: J. Lederberg
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-3,
galT1,
hns-1,
rfbC1,
fic-1,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- fic-1-- Found in W677 and its derivatives. Mutant phenotype is filamentation in the presence of cAMP.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- hns-1 was formerly called osmZ1
- hns-1 was formerly called cur-1
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Lederberg, J. 1950. The selection of genetic recombinations with bacterial growth inhibitors. J.Bacteriol. 59:211-215
- Cavalli, L., G.A. Maccacaro 1950. Chloromycetin resistance in E. coli, a case of quantitative inheritance in bacteria. Nature 166:991-992
- Lederberg, J. 1951. Prevalence of Escherichia coli strains exhibiting genetic recombination. Science 114:68-69
- Lederberg, J., E.M. Lederberg 1951. Recombination analysis of bacterial heredity. Cold Spr.Harb.Symp.Quant.Biol. 16:413-443
- Lederberg, J. 1951. Streptomycin resistance: a genetically recessive mutation. J.Bacteriol. 61:549-550