CGSC Strain#: 5738
Strain Designation: RFS859     
Source of Strain: R. Schleif
Sex: F-
Chromosomal Markers: thr-1,
araC859,
leuB6(Am)
,
Δ(codB-lacI)3,
tsx-274,
λ-,
gyrA111(NalR)
,
recA11,
relA1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- NalR = Nalidix.acidResist.
References: - Schleif, R 1972. Fine-structure deletion map of the Escherichia coli L-arabinose operon. Proc. Natl. Acad. Sci. U.S.A. 69(11):3479-84.