CGSC Strain#: 6592
Strain Designation: pLC16-37/WL8      Source of Strain: J. Walsh
Sex: F- Episome/Plasmid: pLC16-37
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, melA
   Plasmid Comment: Use CGSC No. 6592. Hybridizes with Kohara clones 645-646.
Chromosomal Markers: recA56, srlD300::Tn10, relA1, spoT1, metB1, melA7
Strain Comments:

• The plasmid carries the gene melA.
• recA56-- Amino acid substitution Arg 60 Cys
• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• srlD300::Tn10 was formerly called srlC300::Tn10
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

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