CGSC Strain#: 6600
Strain Designation: CS1230     
Source of Strain: C.A. Schnaitman
Sex: F-
Chromosomal Markers: λ-,
ompC161,
atoS298::Tn10,
gyrA261(NalR)
,
rph-1Strain Comments: - Tn 10 cotransduces 77% with ompC and 30% with gyrA.
- atoS298::Tn10-- : insertion shown by PCR (Nichols et al. 1998) to be within the atoS gene, at nucleotide 2,318,294 (49.972 min.). Originated in Schnaitman strain CS1230.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- atoS298::Tn10 was formerly called zeg-298::Tn10
- atoS298::Tn10 was formerly called zeh-298::Tn10
- atoS298::Tn10 was formerly called zei-298::Tn10
- = tetracycline resistant
- NalR = Nalidix.acidResist.
References: