CGSC Strain#: 5842
Strain Designation: JM1099     
Source of Strain: M.C. Jones-Mortimer
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
galK2(Oc)
,
λ-,
ompF627(T2R)
,
ptsG23,
manXYZ-18,
Δ(his-gnd)79,
mgl-50,
fruA10,
fadL701(T2R)
,
relA1,
thyA111,
pitA10,
spoT1Strain Comments: - galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- manXYZ-18-- : The ptsM locus is now manX,Y, or Z.
- thyA111-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- galK2(Oc) was formerly called gal2
- manXYZ-18 was formerly called ptsM8
- Oc = ochre(UAA) mutation
- T2R = T2 Resistant
References: - Henderson, PJ, RA Giddens, MC Jones-Mortimer 1977. Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. Biochem. J. 162(2):309-20.