CGSC Strain#: 7025
Strain Designation: DB1316     
Source of Strain: D. Botstein
Sex: F-
Chromosomal Markers: λ-,
recD1014(Nuc-)
,
mcrB1,
hsdR2,
opgB202::Tn10Strain Comments: - opgB202::Tn10-- cotransduces 34% with serB and 13% with thr and ca. 50% with hsd. Shown by PCR analysis (Nichols et al.) to be within opgB at nucleotide 4,595,002 (99.0 min.).
- opgB202::Tn10 was formerly called
- = tetracycline resistant
- Nuc- = nuclease-minus
References: - Wertman, K.F., A.R. Wyman, D. Botstein 1986. Host/vector interactions which affect the viability of recombinant phage lambda clones. Gene 49:253-262