CGSC Strain#: 7778
Strain Designation: K1512      Source of Strain: Russel & Model
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Episome/Plasmid: pLW38
   Plasmid Markers/Mutations: bla(ApR), pspAp-lacZ
   Plasmid Comment: This plasmid was made by cloning the {\it pspA} promoter into the {\it SmaI} site of plasmid pSKS107
Chromosomal Markers: garB10, fhuA22, lacIp-4000(lacI^Q), phoA4(Am), ompF627(T2R), Δ(pspA-pspC)637, fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Am = amber(UAG) mutation
• Const = constitutive
• T2R = T2 Resistant
• ApR = ampicillin resistant

References:

• Weiner, L., J.L. Brissette, P. Model 1991. Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on sigma 54 and modulated by positive and negative feedback mechanisms. Genes Dev. 5:1912-1923