CGSC Strain#: 7793
Strain Designation: K1037     
Source of Strain: Russel & Model
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Episome/Plasmid: N3
Plasmid Markers/Mutations: bla(ApR)
,
Tc(tetR)
,
Sm(strR)
,
Sl(sulR) Plasmid Comment: Conjugative IncN plasmid encodes rigid type N pili (phage Ike binding site)
Chromosomal Markers: garB10,
fhuA22,
phoA4(Am)
,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Am = amber(UAG) mutation
- Const = constitutive
- T2R = T2 Resistant
- ApR = ampicillin resistant
- strR = streptomycin resist.
- sulR = sulphonamide resistance
- tetR = tetracycline resistant
References: