CGSC Strain#: 8253
Strain Designation: NCM526     
Source of Strain: N. Majdalani
Sex: F-
Chromosomal Markers: Δ(lacA-lacZ)879(::kan),
lacIp-4000(lacIQ),
zah-2225::FRT,
λ-,
rph-1Strain Comments: - Δ(lacA-lacZ)879(::kan)-- extends from the -35 of lacZ through the stop codon of lacA. The kan cassette is flanked by FRT sites
- zah-2225::FRT-- is the same as zah-2224::cat with the cat cassette removed
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
References: