CGSC Strain#: 5637
Strain Designation: L8     
Source of Strain: D.F. Silbert
Other Designations: LA2-22
Sex: F-
Chromosomal Markers: araC14,
lacY1,
tsx-57,
glnX44(AS)
,
gltA5,
galK2(Oc)
,
Rac-0,
accB22(ts)
,
rpsL20(strR)
,
xylA5,
mtl-1,
lldD1,
thiE1,
tfr-5Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- accB22(ts) was formerly called fabE22
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Harder, M.E., I.R. Beacham, J.E. Cronan, K. Beacham, J.L. Honegger, D.F. Silbert 1972. Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc.Natl.Acad.Sci.USA 69:310
- Silbert, D.F., T. Pohlman, A.B. Chapman 1976. Partial characterization of a temperature-sensitive mutation affecting acetyl coenzyme A carboxylase in Escherichia coli K-12. J.Bacteriol. 126:1351-1354