CGSC Strain#: 6112
Strain Designation: KH6402     
Source of Strain: Imai & Shigesada
Sex: F-
Chromosomal Markers: thr-33,
λ-,
tyrT666(ts,AS)
,
trpE9829(Am)
,
tyrA15(Am)
,
thyA707,
IN(rrnD-rrnE)1,
ilv-683,
metE46,
argH0Strain Comments: - We are not yet able to assign an allele number to the argH allele.
- thyA707-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- ts = Temperature Sensitive
References: - Imai, M, K Shigesada 1978. Studies on the altered rho factor in a nitA mutants of Escherichia coli defective in transcription termination. I. Characterization and quantitative determination of rho in cell extracts. J. Mol. Biol. 120(4):451-66.