CGSC Strain#: 8279
Strain Designation: MC1060/pWTZ594      Source of Strain: J.E. Wertz
Sex: F- Episome/Plasmid: pWTZ594
   Plasmid Markers/Mutations: bla(ApR), lacZp-GFPuv(gfp), ori[pMB1]
   Plasmid Comment: This plasmid was constructed by fusing the GFPuv gene behind the lacZ promoter in pBluescript KS+
Chromosomal Markers: Δ(codB-lacI)3, galK16, galE15(GalS), λ^-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• galK16-- An IS2 insertion resulting in the truncation of the protein at codon 56
• galE15(GalS)-- is Brenner's gal^-_U38, isolated by UV mutagenesis from his strain CA1.
• galE15(GalS)-- G to A transition resulting in an S122F amino acid substitution
• e14--- : Loss of the e14 element makes the strain McrA^-.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• GalS = UDPGal accum.toxic if GalKRP+
• strR = streptomycin resist.
• ApR = ampicillin resistant
• gfp = green fluorescent protein

References:

• Casadaban, MJ, SN Cohen 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138(2):179-207.