CGSC Strain#: 1206
Strain Designation: AB1206      Source of Strain: A.J. Pittard
Sex: F' Episome/Plasmid: F14
   Plasmid Markers/Mutations:
Chromosomal Markers: Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), hisG4(Oc), rpsL8, Δ(ilvE-argH)2, thiE1, tfr-3
Strain Comments:

• References are to F14. The Glansdorff paper is a correction of earlier description.
• This is a primary F' strain.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(ilvE-argH)2-- is the deletion corresponding to the insert in F14.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• PITTARD, J, JS LOUTIT, EA ADELBERG 1963. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J. Bacteriol. 85:1394-401.
• PITTARD, J, EA ADELBERG 1963. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. II. INTERACTION BETWEEN F-MEROGENOTE AND CHROMOSOME DURING TRANSFER. J. Bacteriol. 85:1402-8.
• PITTARD, J, T RAMAKRISHNAN 1964. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI. IV. EFFECT OF A CHROMOSOMAL DELETION ON CHROMOSOME TRANSFER. J. Bacteriol. 88:367-73.
• PITTARD, J, EA ADELBERG 1964. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. III. AN ANALYSIS OF THE RECOMBINATION EVENTS OCCURRING IN THE F' MALE AND IN THE ZYGOTES. Genetics 49:995-1007.
• Glansdorff, N 1967. Pseudoinversions in the chromosome of Escherichia coli K-12. Genetics 55(1):49-61.
• Otsubo, E, HJ Lee, RC Deonier, N Davidson 1974. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. VI. Mapping of F14 sequences homologous to phi 80dmetBJF and phi 80dargECBH bacteriophages. J. Mol. Biol. 89(4):599-618.
• Lee, HJ, E Otsubo, RC Deonier, N Davidson 1974. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ Deletion mutants of F14. J. Mol. Biol. 89(4):585-97.
• Otsubo, E, RC Deonier, HJ Lee, N Davidson 1974. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IV. The F sequences in F14. J. Mol. Biol. 89(4):565-84.