AB2277      Source of Strain:
F- Chromosomal Markers: araBAD-9
, lacY1 or lacZ4
, rpsL8 or rplL9
, thiE1Strain Comments:
- This strain probably carries rfbD1.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- ilvD145-- is suppressed by supH11.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- Eggertsson, G., E.A. Adelberg 1965. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics 52:319-340
- Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194