CGSC Strain#: 4212
Strain Designation: χ478     
Source of Strain: R. Curtiss
Other Designations: chi478
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
secA206(aziR)
,
fhuA23,
lacZ36,
proC32,
tsx-67,
purE42,
glnX44(AS)
,
λ-,
trpE38,
lysA23,
rpsL109(strR)
,
xylA5,
mtl-1,
metE70,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal- phenotype, sup-78,near his.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- aziR = azide resistance
- strR = streptomycin resist.
References: - CURTIS, SR 1965. CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI. J. Bacteriol. 89:28-40.
- Berg, CM, R Curtiss 1967. Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics 56(3):503-25.