CGSC Strain#: 6215
Strain Designation: KL664     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: thr-1,
serR-??-35,
Δ(gpt-proA)62,
glnX44(AS)
,
serS15(ts)
,
rpsL31(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - This strain is temperature-resistant at 42 degrees and temperature-sensitive at 45 degrees C on Luria agar.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- serS15(ts)-- is a temperature-sensitive mutation.
- Δ(gpt-proA)62 was formerly called proA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Theall, G., K.B. Low, D. Soll 1979. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase. Mol.Gen.Genet. 169:205-211