CGSC Strain#: 6336
Strain Designation: HI36      Source of Strain: N. Otsuji
Other Designations: BE5036
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ^-, ruv-9, hisG4(Oc), rpsL31(strR), xylA5, mtlA2, argE3(Oc), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Otsuji, N, H Iyehara, Y Hideshima 1974. Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation. J. Bacteriol. 117(2):337-44.
• Iyehara, H, N Otsuji 1975. Location of the Escherichia coli K-12 ruv gene affecting septum formation after inhibition of deoxyribonucleic acid synthesis. J. Bacteriol. 122(2):791-3.
• Shurvinton, C.E., R.G. Lloyd, F.E. Benson, P.V. Attfield 1984. Genetic analysis and molecular cloning of the Escherichia coli ruv gene. Mol.Gen.Genet. 194:322-329