CGSC Strain#: 6345
Strain Designation: RS3116     
Source of Strain: R.W. Simons
Sex: F-
Chromosomal Markers: lacY1 or lacZ4,
glnX44(AS)
,
aroC4,
fadL701(T2R)
,
dsdA1,
zfd-1::Tn10,
rpsL8 or rplL9(L?)
or rpsL14,
malT1(λ
R)
,
xyl-7,
mtlA2,
ilvD188(Oc)
,
thiE1,
tsx-23 or tsx-25Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- dsdA1-- is called dsdA7 by McFall (1967 Genetics 55:91).
- zfd-1::Tn10-- cotransduces 80% with dsdA, 35% with fadL and 20% with aroC. PCR analysis places the insertion between orf b2374 and orf b2375, at nucleotide 2,491,451 (53.7 min.)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- = tetracycline resistant
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- T2R = T2 Resistant
References: